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Original Research Article | OPEN ACCESS

1, 25-Dihydroxyvitamin D3 suppresses cell cycle progression and thus growth of prostate cancer cells by inducing expression of limb bud and heart development (LBH)

Heng Du1, Jing Zhuang2, Yaping Gui3, Qiuyue Xu4, Li Zhu5, Le Gao1, Jie Jiang5

1Department of Urology, Second Affiliated Hospital of Xinjiang Medical University, Xinjiang 860063; 2Department of Nephrology, People's Hospital of Xinjiang Uygur Autonomous Region,Urumqi, 830000; 3Tongji Hospital Affiliated Tongji University in Shanghai, Shanghai, 200000; 4Nanjing University of Chinese Medicine, Nanjing 210023; 5Department of Urology, Affiliated Hospital of Changchun University of Traditional Chinese Medicine, Changchun, 130021, China.

For correspondence:-  Jie Jiang   Email: jiangjiemedcch@163.com

Accepted: 26 August 2018        Published: 30 September 2018

Citation: Du H, Zhuang J, Gui Y, Xu Q, Zhu L, Gao L, et al. 1, 25-Dihydroxyvitamin D3 suppresses cell cycle progression and thus growth of prostate cancer cells by inducing expression of limb bud and heart development (LBH). Trop J Pharm Res 2018; 17(9):1733-1738 doi: 10.4314/tjpr.v17i9.7

© 2018 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To investigate the function of limb bud and heart development (LBH) in 1α, 25-dihydroxyvitamin D3 (1,25D)-mediated inhibitory effect on proliferation of prostate cancer cells.
Methods: The inhibitory effect of 1,25D on growth and cell cycle progression of lymph node carcinoma of the prostate (LNCaP) cells was determined using cell counting kit-8 (CCK-8) assay, 5-ethynyl-2'-deoxyuridine (EdU) assay and flow cytometry, while the expression levels of LBH in response to treatment with 1,25D were determined by quantitative reverse-transcription PCR (qRT-PCR) and western blottingting. The expression levels of LBH in cells were down/up regulated by transfection with siRNA or overexpression plasmids, and then cell growth and cell cycles were measured using the CCK-8 assay, EdU assay, and flow cytometry. Finally, the growth inhibitory effect of 1,25D on LBH knockdown cells were determined using CCK-8 and EdU assays.
Results: Treatment with 1,25D arrested LNCaP cells in G0/G1 phase of cell cycle, suppressed the growth of the cells and induced the expression of LBH. Overexpression/knockdown of LBH in LNCaP cells suppressed/promoted cell growth and accumulated/decreased cells in the G0/G1 phase. Moreover, knockdown of LBH reversed the inhibitory effect of 1,25D on cell proliferation of LNCaP cells.
Conclusion: Inhibitory effect of 1,25D on cell cycle progression and cell proliferation might be via LBH.

Keywords: Cell proliferation, Limb bud and heart development, Prostate cancer, 1^5;, 25-Dihydroxyvitamin D3

Impact Factor
Thompson Reuters (ISI): 0.523 (2021)
H-5 index (Google Scholar): 39 (2021)

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